Construction of Multi-Epitopes Vaccine Candidate against SARS-CoV-2 D614G Variant

COVID-19 caused by the SARS-CoV-2 virus has become a real threat due to emergence of new variants which are more deadly with higher infectivity. Vaccine constructs that target specific needed for stemming fatality. The spike (S) glycoprotein is major antigenic component triggers host immune response. Reverse vaccinology strategy was applied S protein variant D614G identify highly ranked proteins. In this study, multi-epitope synthetic gene designed using computational strategies variant. sequence retrieved from NCBI database. prediction linear B-cell epitopes carried out Artificial Neural Network (ANN)-based ABCpred and BepiPred 2.0 software. top 15 sequences were then selected. Propred 1 servers used histocompatibility complex (MHC) class I II binding within pre-determined predict T-cell epitopes. 5 MHC Further in-silico testing its solubility, allergenicity, antigenicity, other physiochemical properties analyzed Bpred. constructed subjected assembly PCR product confirmed Sanger sequencing. findings study suggested region can be predicted amplified method. shown elicit humoral cell-mediated responses towards variants.